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KanR-BA-Bs

Part:BBa_K349008:Design

Designed by: David Lloyd   Group: iGEM10_Alberta   (2010-10-21)

KanR-BA-BsaI (for construction of BA BioBytes 2.0)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 984


Design Notes

This part has built-in BsaI cutsites surrounding the p1003 kanamycin resistance cassette. Digestion at these sites produces a B overhang at the 5' end and an A overhang at the 3' end consistent with the Biobytes 2.0 Assembly Method.

Source

pSB1C3

References